The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition. Tested in polyacrylamide gel electrophoresis and Western Blotting. It offers a broad coverage of protein sizes ranging from 15. One prominent feature of this protein ladder, is that it remains in the liquid state at the storage temperature therefore, there is not a freeze-thaw cycle involved, making the ladder very stable.Ġ.1~0.4 mg/ml of each protein in 20 mM Trisphosphate (pH 7.5 at 25☌), 2% SDS, 1 mM reducing reagent, 3.6 M Urea, and stabilizer.įor each application, 5-15µl is needed, depending on the size of the wells. It is used as protein size markers in immuno-detection in Western blot on on film or by CCD imaging. No need to heat, dilute, or add reducing agent before loading. The ladder is supplied in protein gel loading buffer and is ready-to-use. It is designed for monitoring protein separated during polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes and for sizing of proteins. The Bullseye Pre-stained Protein Ladder contains 10 prestained proteins, covering a wide range of molecular weights from 10 to 180kDa. Quality Control : 5 l of BLUltra Prestained Protein Ladder (PM001-0500) resolves 10 bands in 4-20 SDS-PAGE (Tris-glycine buffer) and after Western blotting to. Use 5-15µl, depending on the size of the wellsĪpplication: The Bullseye Pre-stained Protein Ladder is suitable for visualizing proteins during electrophoresis without staining and for monitoring transfer onto membrane.The protein standard is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce. 2 reference bands - red at ~75kDa and green at ~25kDa Thermo Scientific Spectra Multicolor Low Range Protein Ladder is a mixture of six proteins and synthetic peptides (1.7 to 40 kDa) for use as size standards for peptides and small proteins in gel electrophoresis and western blotting.Stoolfix Gut Microbiome Stabilization Kitsīullseye Pre-stained Protein Ladder, 10-180 kDa In western blotting, prestained protein ladders can be used to: Monitor protein separation during SDS-polyacrylamide gel electrophoresis Verify transfer.Beakers, Graduated Cylinders, and Flasks.Aluminum Foil, Parafilm, and Multiwipes.
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